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Data on comparative proteomic profiling of human sperm affected by 4-tert-octylphenol in vitro

Shaoping Huang1,2, Senyang Cao2,3, Tao Zhou2,4

  • 1Department of Histology and Embryology, Medical School, Southeast University, Nanjing 210009, Jiangsu, China.

Data in Brief
|December 18, 2018
PubMed

Insights

This study reveals how 4-tert-octylphenol (4t-OP) damages human sperm motility and viability. Proteomic analysis identified key protein changes and posttranslational modifications affected by 4t-OP exposure.

Area of Science:

  • Reproductive biology
  • Environmental toxicology
  • Proteomics

Background:

  • Sperm motility and viability are crucial for male fertility.
  • Environmental phenols, such as 4-tert-octylphenol (4t-OP), are emerging contaminants with potential reproductive toxicity.
  • Previous research indicated 4t-OP affects human sperm function via signaling pathways.

Purpose of the Study:

  • To present comprehensive proteomic profiling of human sperm exposed to 4t-OP.
  • To identify differentially expressed proteins and their posttranslational modifications (PTMs).
  • To elucidate the molecular mechanisms underlying 4t-OP-induced sperm damage.

Main Methods:

  • Comparative proteomics analysis of human sperm treated with varying concentrations of 4t-OP (0, 0.1, 0.3 mM) in vitro.
  • Mass spectrometry was employed to identify proteins and PTMs.
  • Differentially expressed proteins were mapped to the human sperm proteome 2.0 database.

Main Results:

  • Identification of specific proteins and PTMs altered by 4t-OP exposure.
  • Detailed data on PTMs in proteins related to apoptosis and motility.
  • Raw mass spectrometry files and PTM data are provided for further research.

Conclusions:

  • 4t-OP exposure leads to significant changes in sperm proteome.
  • These proteomic alterations provide insights into the molecular basis of impaired sperm motility and viability.
  • The data supports the link between 4t-OP, cAMP-PKA/PKC signaling, and tyrosine phosphorylation in sperm dysfunction.

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