Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Power01:08

Power

13.0K
The concept of work involves force and displacement; meanwhile, the work-energy theorem relates the net work done on a body to the difference in its kinetic energy, calculated between two points on its trajectory. While none of these quantities or relations involves time explicitly, we know that the time available to accomplish work is often just as important as the amount of work itself. For example, sprinters in a race may have achieved the same velocity at the finish, therefore,...
13.0K
Nuclear Power02:36

Nuclear Power

9.5K
Controlled nuclear fission reactions are used to generate electricity. Any nuclear reactor that produces power via the fission of uranium or plutonium by bombardment with neutrons has six components: nuclear fuel consisting of fissionable material, a nuclear moderator, a neutron source, control rods, reactor coolant, and a shield and containment system.
Nuclear Fuels
Nuclear fuel consists of a fissile isotope, such as uranium-235, which must be present in sufficient quantity to provide a...
9.5K
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

649
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
649
Molecules and Compounds02:38

Molecules and Compounds

68.7K
Atoms and Molecules
68.7K
Structure of Lipids03:38

Structure of Lipids

98.7K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
98.7K
Instantaneous Power01:22

Instantaneous Power

914
Instantaneous power is important in electrical circuits, mainly when dealing with sinusoidal input. Instantaneous power, denoted as p(t), results from the multiplication of the instantaneous voltage (v(t)) across an element and the instantaneous current (i(t)) flowing through it. This relationship adheres to the passive sign convention and represents a fundamental principle in electrical engineering.
914

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolution of Strategies Toward the Total Synthesis of Aleutianamine.

The Journal of organic chemistry·2026
Same author

Glass Cannula for Bulb-to-Bulb Cold Transfer.

Organic syntheses; an annual publication of satisfactory methods for the preparation of organic chemicals·2026
Same author

Radical Deoxygenation of Alcohols via Visible Light Irradiation of a Titanium Porphyrin Catalyst.

Organic letters·2026
Same author

Stealing from a distant neighbor: an unexpectedly fast long-span peroxy radical hydrogen-shift reaction in a long-chain diether.

Chemical science·2026
Same author

Synthesis of 6-<i>epi</i>-Hypersampsone R by a Desymmetrization Strategy.

Organic letters·2026
Same author

Enantioselective Michael Spirocyclization of Palladium Enolates.

ACS catalysis·2026

Related Experiment Video

Updated: Feb 1, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.8K

The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination.

Christopher G Jones1, Michael W Martynowycz1, Johan Hattne1

  • 1Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, Departments of Biological Chemistry and Physiology, and UCLA-DOE Institute, University of California, Los Angeles, California 90095, United States.

ACS Central Science
|December 18, 2018
PubMed
Summary
This summary is machine-generated.

Microcrystal electron diffraction (MicroED) offers routine, unambiguous small molecule structure determination. This electron cryo-microscopy (cryoEM) method rapidly provides atomic resolution from nanocrystals, overcoming limitations of traditional crystallography.

More Related Videos

gP2S, an Information Management System for CryoEM Experiments
13:01

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

5.9K
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
04:50

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

Published on: February 8, 2022

6.6K

Related Experiment Videos

Last Updated: Feb 1, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.8K
gP2S, an Information Management System for CryoEM Experiments
13:01

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

5.9K
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
04:50

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

Published on: February 8, 2022

6.6K

Area of Science:

  • Organic Chemistry
  • Structural Biology
  • Materials Science

Background:

  • Unambiguous identification of small molecules is crucial in organic chemistry.
  • Traditional methods like NMR spectroscopy infer connectivity, while X-ray diffraction provides definitive structures.
  • X-ray crystallography faces limitations in routine application due to analyte and technique constraints.

Purpose of the Study:

  • To introduce microcrystal electron diffraction (MicroED) as a routine method for unambiguous small molecule structure determination.
  • To demonstrate the capability of MicroED to rapidly obtain atomic resolution structures from minimal sample amounts.

Main Methods:

  • Utilized electron cryo-microscopy (cryoEM) with microcrystal electron diffraction (MicroED).
  • Processed simple organic powders with minimal sample preparation.
  • Collected high-quality MicroED data from nanocrystals (approximately 100 nm).

Main Results:

  • Achieved routine and unambiguous structural determination of small organic molecules.
  • Obtained atomic resolution (<1 Å) crystal structures in minutes.
  • Demonstrated success with nanocrystals as small as ~10-15 grams.

Conclusions:

  • MicroED provides a powerful alternative to traditional methods for small molecule structural analysis.
  • This technique significantly accelerates the structure determination process in organic chemistry.
  • MicroED overcomes key limitations of X-ray crystallography for routine small molecule analysis.