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Updated: Jan 31, 2026

Evaluation of the Storage Stability of Extracellular Vesicles
Published on: May 22, 2019
Development and evaluation of Chrysin-Phospholipid complex loaded solid lipid nanoparticles - storage stability and
Shahadali Komath1, Anuj Garg1,2, Muhammad Wahajuddin3
1a Department of Pharmaceutics , National Institute of Pharmaceutical Education and Research (NIPER) , Raebareli , Uttar Pradesh , India.
Context:
Flavonoids show promising anticancer potential but it is limited due to poor solubility.
Objective:
The present investigation was to prepare Chrysin-Phospholipid complex loaded solid lipid nanoparticles (Ch-PC-SLNs) for improving its encapsulation as compared to that of Chrysin loaded SLNs (Ch-SLNs) and evaluated for potential increase in the anti-cancer activity against MCF-7 cell line.
Methods:
The physiochemical characteristics and release kinetics for Ch-SLNs and Ch-PC-SLNs were evaluated and compared. Storage stability of Ch-PC-SLNs was evaluated up to 3 months. Solid state properties (DSC, XRD) and morphology (AFM) of Ch-PC-SLNs were also studied. In-vitro anticancer activity was investigated by using MTT assay.
Results And Discussion:
Ch-PC-SLNs exhibited higher encapsulation efficiency than Ch-SLNs and zero order release kinetics. Ch-PC-SLNs were found to be stable upto 3 months upon lyophilisation with mannitol as cryoprotectant. DSC and XRD study revealed the loss of highly crystalline nature of Chrysin in Ch-PC-SLNs. The Ch-PC-SLNs lead to significantly higher in-vitro anticancer activity than that of bulk Chrysin.
Conclusion:
The study concludes that phospholipids complex with Chrysin lead to improve encapsulation, storage stability of SLNs and in vitro anticancer activity.
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