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Updated: Jan 31, 2026

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers
Published on: March 3, 2023
Cryopreserved amniotic membrane in the treatment of diabetic foot ulcers: a case series
Mónica Rodríguez Valiente1, Francisco J Nicolás2, Ana M García-Hernández3
1Cell Therapy Unit at Hospital Clínico Universitario Virgen de la Arrixaca. Biomedical Research Institute of Murcia (Instituto Murciano de Investigación Biosanitaria, IMIB-Arrixaca). El Palmar, Spain; and Department of General Surgery, Hospital Clínico Universitario Virgen de la Arrixaca. Biomedical Research Institute of Murcia (Instituto Murciano de Investigación Biosanitaria, IMIB-Arrixaca). El Palmar, Spain.
Objective:
The amniotic membrane (AM) is a tissue with low immunogenity and high therapeutic potential due to its anti-inflammatory, anti-fibrotic and antimicrobial effects. This paper describes the use of cryopreserved amniotic membrane allografts to treat diabetic foot ulcers (DFUs) in patients with diabetes.
Method:
In this case series, AM was processed to obtain a final medicinal product: cryopreserved amniotic membrane. cryopreserved AM was applied every 7-10 days until total epithelialisation of the DFUs.
Results:
A total of 14 patients with DFUs (median size: 12.30cm, (range: 0.52-42.5cm2) were treated and followed up until complete closure (median time: 20 weeks, range: 7-56 weeks). Patients received 4-40 AM applications. All patients in this study achieved complete epithelialisation of the wound. No adverse events were observed.
Conclusion:
AM is a feasible and safe treatment in complex DFUs. Furthermore, the treatment is successful in achieving epithelialisation of long-evolution, unhealed wounds resistant to conventional therapies.
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