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Expression of the Short Form of RON/STK in Feline Mammary Carcinoma

Lorella Maniscalco1, Silvia Guil-Luna2,3, Selina Iussich1

  • 11 Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.

Veterinary Pathology
|December 19, 2018
PubMed

Insights

Researchers studied feline mammary carcinoma (FMC) and found the short form of RON (sf-RON) is expressed in tumors. sf-RON presence indicates a poorer prognosis, supporting FMC as a comparative oncology model.

Area of Science:

  • Oncology
  • Molecular Biology
  • Veterinary Medicine

Background:

  • The RON receptor tyrosine kinase and its ligand, macrophage-stimulating protein (MSP), are implicated in human breast cancer progression.
  • The short form of RON (sf-RON) arises from an alternative promoter and plays a role in human breast cancer.
  • Feline mammary carcinoma (FMC) shares similarities with human breast cancer, making it a valuable model for comparative studies.

Purpose of the Study:

  • To investigate the expression of RON and MSP in feline mammary tumors.
  • To identify the presence and origin of the sf-RON transcript in FMCs.
  • To evaluate the association of sf-RON expression with tumor characteristics and patient outcomes.

Main Methods:

  • Immunohistochemistry was used to detect RON and MSP expression in 50 FMCs and 10 benign lesions.
  • RNA was extracted from paraffin-embedded tissues for RT-PCR analysis to identify sf-RON transcripts.
  • Statistical analysis, including hazard ratios, was performed to assess the impact of sf-RON on disease-free period and survival.

Main Results:

  • RON and MSP expression was detected in 68% and 58% of FMCs, respectively.
  • The sf-RON transcript was identified in 38% of FMCs, originating from an alternative promoter (P2) as seen in humans.
  • sf-RON expression correlated with poorly differentiated tumors, shorter disease-free periods (HR, 2.2), and reduced survival (HR, 2.2).

Conclusions:

  • Feline mammary carcinoma serves as a relevant model for comparative oncology research.
  • The expression of sf-RON in FMCs is linked to adverse prognostic factors.
  • sf-RON may serve as a potential predictive biomarker for outcomes in feline mammary carcinoma.
Keywords:
catscomparative oncologyfeline mammary tumorsprognosisshort form of RON

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