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Updated: Jan 31, 2026
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Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury
Published on: February 23, 2014
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Systemic functional enrichment and ceRNA network identification following peripheral nerve injury
Tianmei Qian1, Chunlin Fan2, Qianyan Liu1
1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu Province, People's Republic of China, 226001.
Molecular Brain
|December 19, 2018
Summary
Peripheral nerve injury impairs regeneration. This study reveals dynamic mRNA and lncRNA changes, identifying a ceRNA network crucial for understanding nerve repair mechanisms.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Peripheral nerve injury presents a significant global health challenge, impacting patient quality of life and imposing societal economic burdens.
- While peripheral nerves possess intrinsic regenerative capabilities, severe injuries severely limit functional recovery.
- Understanding the molecular mechanisms underlying nerve injury is critical for developing effective clinical treatments.
Purpose of the Study:
- To systematically explore dynamic changes in messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) in injured sciatic nerve segments.
- To identify key Gene Ontology (GO) terms and Kyoto Enrichment of Genes and Genomes (KEGG) pathways involved in peripheral nerve injury.
- To analyze the correlation between differentially expressed mRNAs and lncRNAs and construct a competing endogenous RNA (ceRNA) network.
Main Methods:
- Sciatic nerve crush model in rodents.
- High-throughput sequencing of mRNAs and lncRNAs.
- Bioinformatic analysis including clustering, GO, and KEGG pathway enrichment.
- Construction of a ceRNA network focusing on the "negative regulation of cell proliferation" GO term.
Main Results:
- Identified dynamic changes in mRNA and lncRNA expression profiles following sciatic nerve crush.
- Determined significantly enriched GO terms and KEGG pathways related to nerve injury and regeneration.
- Established a novel mRNA-miRNA-lncRNA ceRNA network involving LIF and HMOX1 genes within the "negative regulation of cell proliferation" pathway.
Conclusions:
- This study provides a comprehensive analysis of the dynamic transcriptome and lncRNA landscape after peripheral nerve injury.
- The identified ceRNA network offers new insights into the regulatory mechanisms governing nerve repair.
- This foundational work paves the way for future research into the role of non-coding RNAs in peripheral nerve regeneration.
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