[Detection methods of genome editing in plants]

Area of Science:

• Genetics
• Molecular Biology
• Plant Science

Background:

• CRISPR/Cas9 technology revolutionizes genome editing in living organisms, accelerating genetic engineering and plant molecular breeding.
• The rapid expansion of genome-edited plants necessitates efficient, high-throughput screening methods for early-stage identification.

Purpose of the Study:

• To review and compare molecular methods for identifying genome-edited plants.
• To analyze the advantages, disadvantages, and applications of various detection techniques.
• To provide insights into future trends in plant genome editing detection.

Main Methods:

• Literature review of recent molecular identification techniques for genome-edited plants.
• Comparative analysis of different methods based on efficiency, throughput, and applicability.
• Discussion of emerging trends and future directions in detection technologies.

Main Results:

• Several molecular methods have been developed for identifying genome-edited plants.
• Each method possesses unique strengths and limitations impacting its suitability for different research scales.
• The choice of method depends on factors like throughput requirements, cost, and desired precision.

Conclusions:

• Efficient screening is crucial for advancing plant genome editing and molecular breeding.
• Continued development of rapid and high-throughput detection methods is essential.
• This review serves as a valuable reference for researchers in plant genome editing.