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Updated: Jan 31, 2026

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
Control freaks-signals and cues governing the regulation of virulence in attaching and effacing pathogens
Natasha C A Turner1, James P R Connolly1, Andrew J Roe2
1Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow G12 8TA, U.K.
Abstract:
Enterohaemorrhagic Escherichia coli (EHEC) mediates disease using a type 3 secretion system (T3SS), which is encoded on the locus of enterocyte effacement (LEE) and is tightly controlled by master regulators. This system is further modulated by a number of signals that help to fine-tune virulence, including metabolic, environmental and chemical signals. Since the LEE and its master regulator, Ler, were established, there have been numerous scientific advancements in understanding the regulation and expression of virulence factors in EHEC. This review will discuss the recent advancements in this field since our previous review, with a focus on the transcriptional regulation of the LEE.
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