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Imaging cellular ultrastructures using expansion microscopy (U-ExM).

Davide Gambarotto1, Fabian U Zwettler2, Maeva Le Guennec1

  • 1Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland.

Nature Methods
|December 19, 2018
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Summary
This summary is machine-generated.

Ultrastructure expansion microscopy (U-ExM) visualizes biological ultrastructures with optical microscopy. This technique reveals fine details previously only visible with electron microscopy.

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Macromolecular assemblies are crucial for cellular function.
  • Determining their structure and composition is a significant biological challenge.
  • Existing methods have limitations in visualizing fine ultrastructural details.

Purpose of the Study:

  • To introduce ultrastructure expansion microscopy (U-ExM).
  • To enable visualization of preserved ultrastructures using optical microscopy.
  • To achieve near-native expansion of diverse biological structures.

Main Methods:

  • U-ExM is an extension of expansion microscopy.
  • It allows for physical expansion of biological samples.
  • Combined with super-resolution microscopy for enhanced detail.

Main Results:

  • U-ExM enables visualization of preserved ultrastructures via optical microscopy.
  • The method achieves near-native expansion of various structures in vitro and in cells.
  • It revealed intricate ultrastructural organization, including centriolar chirality.

Conclusions:

  • U-ExM provides a powerful tool for studying macromolecular assemblies.
  • It bridges the resolution gap between optical and electron microscopy.
  • This technique offers new possibilities for understanding cellular ultrastructure.