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The First Molecular Detection of Aichi Virus 1 in Raw Sewage and Mussels Collected in South Africa
Oikwathaile Onosi1, Nicole S Upfold2, Michael D Jukes1
1Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, 6140, South Africa.
Abstract:
Aichi virus 1 (AiV-1) has a worldwide distribution and is associated with gastroenteritis in humans. In this study, raw sewage and mussel samples were analyzed for the presence of AiV-1 using reverse transcription-PCR (RT-PCR). Amplification and sequencing of the 3CD and VP1 genomic regions followed by phylogenetic analysis using selected genome sequences revealed the presence of AiV-1, genotype B. The results highlight the importance of further screening to evaluate the prevalence and epidemiology of this clinically important virus in South Africa.
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