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Western Blotting: Sample Preparation to Detection
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Enhancement of luminol-based immunodot and Western blotting assays by iodophenol

M M Leong1, G R Fox

  • 1Department of Biology, University of Victoria, British Columbia, Canada.

Analytical Biochemistry
|July 1, 1988
PubMed

The application of 4-iodophenol as a signal enhancer for luminol-based immunodot and Western blotting assays was investigated. Immunodetection of biotin-bovine serum albumin (BSA) using a rat anti-biotin antibody for immunodot binding and goat anti-biotin peroxidase conjugate for Western blotting assays was employed to demonstrate the signal enhancement attainable with 4-iodophenol. The addition of 4-iodophenol at a final working concentration of 52.2 microM to a light-emitting substrate, luminol, resulted in 3-fold and 10-fold signal enhancements for Western blotting and immunodot binding detection of biotin-BSA, respectively.

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