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What is Cell Signaling?

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Related Experiment Video

Updated: Jan 31, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Dynamic Microfluidic Cytometry for Single-Cell Cellomics: High-Throughput Probing Single-Cell-Resolution Signaling.

Peng Chen¹, Shuangqian Yan¹, Jie Wang¹

¹The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology , Huazhong University of Science and Technology , Wuhan 430074 , China.

Analytical Chemistry

|December 19, 2018

Summary

Dynamic microfluidic cytometry (DMC) enables high-throughput single-cell analysis of G protein-coupled receptor (GPCR) signaling. This new method precisely manipulates cells for detailed dynamic signaling studies.

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Area of Science:

Biotechnology
Cell Biology
Pharmacology

Background:

Cell signaling is crucial for physiological functions but is challenging to study at the single-cell level due to throughput limitations and imprecise fluid handling.
Investigating dynamic cellular responses requires advanced techniques for precise manipulation and high-volume analysis.

Purpose of the Study:

To introduce dynamic microfluidic cytometry (DMC) as a novel strategy for high-throughput, single-cell resolution analysis of G protein-coupled receptor (GPCR) signaling.
To overcome limitations in current methods for studying dynamic cellular processes at the single-cell level.

Main Methods:

Development and application of dynamic microfluidic cytometry (DMC) utilizing cyclical cell trapping, stimulation, and release.
High-throughput single-cell analysis of GPCR signaling pathways, including dose-response curves and EC50 determination.
Implementation of sequential and simultaneous stimulation protocols for dynamic signaling studies in single cells and co-cultures.

Main Results:

Successfully obtained dose-response curves and EC50 values for HeLa cells stimulated with ATP, histamine, and acetylcholine chloride at the single-cell level.
Revealed distinct mechanisms of intracellular calcium release through high-throughput single-cell dynamic signaling analysis.
Enabled online comparison of dynamic signaling between different cell types (HeLa and NIH-3T3) through simultaneous stimulation.

Conclusions:

Dynamic microfluidic cytometry (DMC) offers a versatile platform for high-throughput probing of single-cell dynamic signaling.
The DMC technique has significant potential applications in chemical biology, cell biology, and pharmacology for detailed signaling pathway investigations.