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Isolation and separation of Listeria monocytogenes using bacteriophage P100-modified magnetic particles

Yan Zhou1, Ramaraja P Ramasamy1

  • 1Nano Electrochemistry Laboratory, School of Chemical, Materials and Biomedical Engineering, University of Georgia, Athens, Georgia 30602, USA; Department of Chemistry, University of Georgia, Athens, Georgia 30602, USA.

Colloids and Surfaces. B, Biointerfaces
|December 19, 2018
PubMed

Insights

This study developed a phage-assisted magnetic separation method for isolating Listeria monocytogenes from food. Physical immobilization of bacteriophages on magnetic particles demonstrated superior capture efficiency and selectivity for effective bacterial separation.

Area of Science:

  • Food microbiology
  • Biotechnology
  • Nanotechnology

Background:

  • Accurate detection of Listeria monocytogenes in food is crucial for public health.
  • Existing methods for bacterial isolation can be time-consuming and lack specificity.
  • Bacteriophage-based methods offer a highly specific approach to pathogen detection.

Purpose of the Study:

  • To develop and optimize a bacteriophage-assisted magnetic separation (BAMS) method for Listeria monocytogenes isolation.
  • To investigate the impact of magnetic particle size and immobilization techniques on phage coupling and infectivity.
  • To evaluate the efficiency and selectivity of BAMS for L. monocytogenes in complex food matrices.

Main Methods:

  • Development of bacteriophage P100-modified magnetic particles (PMMPs) using three magnetic particle sizes (150 nm, 500 nm, 1 μm).
  • Immobilization of bacteriophages onto magnetic particles using both chemical and physical methods.
  • Evaluation of phage coupling ratio and L. monocytogenes capture efficiency for different PMMP configurations.
  • Testing of optimized PMMPs for selective isolation of L. monocytogenes from real food samples (ground beef, whole milk).

Main Results:

  • Physically immobilized PMMPs exhibited higher initial capture efficiency compared to chemically immobilized ones.
  • The PMMP complexes demonstrated excellent selectivity towards Listeria monocytogenes.
  • Optimized PMMPs successfully isolated L. monocytogenes from complex food matrices like ground beef and whole milk.

Conclusions:

  • Bacteriophage-assisted magnetic separation is a promising technique for rapid and selective isolation of Listeria monocytogenes from food.
  • Physical immobilization of bacteriophages on magnetic particles enhances capture efficiency.
  • This method holds potential for improving food safety testing protocols.
Keywords:
Bacteriophage P100Capture efficiencyL. monocytogenesListeria enrichmentMagnetic separationPhage immobilization method

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