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Microarray expression profile and analysis of circular RNA regulatory network in malignant pleural effusion
Yakun Wen1, Yong Wang2, Zhenchuan Xing1
1a Department of Respiratory and Critical Care Medicine , Beijing Luhe Hospital, Capital Medical University , Beijing , China.
Cell Cycle (Georgetown, Tex.)
|December 20, 2018
Summary
Circular RNAs (circRNAs) are newly identified in lung adenocarcinoma-associated malignant pleural effusion (LA-MPE). These circRNAs may play a role in LA-MPE development by regulating cancer-related pathways.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Malignant pleural effusion (MPE) frequently complicates lung cancer.
- Circular RNAs (circRNAs) are implicated in cancer development, but their role in MPE is unclear.
Purpose of the Study:
- To investigate the expression patterns and potential roles of circRNAs in lung adenocarcinoma-associated malignant pleural effusion (LA-MPE).
Main Methods:
- Microarray analysis (Clariom D Human Microarray) was used to identify differentially expressed circRNAs and mRNAs in LA-MPE compared to tuberculous pleural effusion (TPE).
- Quantitative real-time PCR (qRT-PCR) was employed to validate the expression of top upregulated circRNAs.
- CircRNA-miRNA-mRNA networks were constructed, and pathway analysis was performed.
Main Results:
- 1350 differentially expressed circRNAs and 1727 differentially expressed mRNAs were identified in LA-MPE versus TPE.
- Four of the top five upregulated circRNAs were validated by qRT-PCR, with hsa_circ_0085386 showing an increasing trend.
- Pathway analysis revealed that aberrantly expressed circRNAs potentially regulate key cancer-related pathways, including PI3K-Akt signaling and Axon guidance.
Conclusions:
- This study provides the first specific circRNA profiles for LA-MPE.
- Aberrantly expressed circRNAs may contribute to LA-MPE development by influencing cancer-related signaling pathways through miRNA sequestration.
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