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Related Experiment Video

Updated: Jan 31, 2026

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
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Immunohistochemistry to Study YAP in Human Tissue Samples.

Franziska Haderk1,2,3, Victor Olivas1,2,3, Trever G Bivona4,5,6

  • 1Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 20, 2018
PubMed
Summary

Immunohistochemistry (IHC) analysis of YAP protein in human tissues is crucial for understanding gene activity. Optimized IHC protocols ensure accurate YAP detection in FFPE samples, aiding in predicting YAP activity and gene transcription states.

Keywords:
Digital pathologyFFPE samplesHippo signalingIHCImmunohistochemistryYAPYAP nuclear activity

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Pathology

Background:

  • YAP (Yes-associated protein) functions as a transcriptional coactivator.
  • Altered YAP nuclear levels predict YAP activity and target gene transcriptional states.
  • Accurate YAP detection in human tissues is vital for research and diagnostics.

Purpose of the Study:

  • To provide optimized Immunohistochemistry (IHC) procedures for YAP detection.
  • To detail essential steps for successful YAP staining in FFPE tissues.
  • To enable reliable analysis of YAP expression and localization.

Main Methods:

  • Immunohistochemistry (IHC) on Formalin-Fixed Paraffin-Embedded (FFPE) human tissue samples.
  • De-paraffinization and heat-induced antigen retrieval are critical steps.
  • Utilizing a labeled polymer-HRP system with diaminobenzidine (DAB) chromogen for signal amplification.

Main Results:

  • Optimized IHC protocols allow for YAP detection in FFPE tissues.
  • Successful staining requires de-paraffinization and heat-induced antigen retrieval.
  • The chosen detection system provides strong staining with minimal background.

Conclusions:

  • The described IHC method is effective for analyzing YAP expression and localization in human FFPE tissues.
  • This protocol facilitates predictions of YAP activity and target gene transcription.
  • The optimized procedure ensures reliable and high-quality YAP IHC staining.