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Updated: Jan 31, 2026

De Novo Generation of Somatic Stem Cells by YAP/TAZ
Published on: May 7, 2018
Immunofluorescence Study of Endogenous YAP in Mammalian Cells
Valentina Rausch1, Carsten G Hansen2
1University of Edinburgh Centre for Inflammation Research, Queen's Medical Research Institute, Edinburgh, UK.
Abstract:
Immunocytochemistry enables determination of cellular localization and relative abundance of proteins. This protocol describes a rapid and cost-effective approach to study the cellular localization of YAP (and TAZ), the transcriptional co activators of the Hippo pathway, in mammalian cells. Cells are seeded onto coated cover slips, cultivated and treated as required. Subsequently, they are chemically fixed, and cellular proteins are fluorescently labeled by means of specific antibodies. Multiplexing antibodies enables ascertaining the subcellular localization of YAP and TAZ and thereby also the activation state of the Hippo pathway in various cell types.
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