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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels.

Ryan J Quinton1, Neil J Ganem2,3

  • 1Department of Pharmacology and Experimental Therapeutics, The Cancer Center, Boston University School of Medicine, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 20, 2018
PubMed
Summary

CRISPR technology enables precise gene editing. Researchers used CRISPR to knock out YAP and TAZ genes, offering new ways to study Hippo signaling pathways.

Keywords:
CRISPRaCRISPRiCas9HippoKRABLATSVP64sgRNA

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Area of Science:

  • Genomic Engineering
  • Molecular Biology
  • Cellular Signaling

Background:

  • CRISPR technology has transformed gene editing capabilities.
  • YAP and TAZ are key transcriptional co-activators in the Hippo signaling pathway.
  • Regulating YAP/TAZ levels is a potential therapeutic strategy.

Purpose of the Study:

  • To describe strategies for using CRISPR to genetically knock out YAP (YAP1) and TAZ (WWTR1) genes.
  • To outline methods for transcriptionally modulating YAP/TAZ levels using CRISPR.
  • To explore the application of CRISPR in Hippo signaling research.

Main Methods:

  • CRISPR-mediated gene knockout in mammalian cell lines.
  • Gene editing strategies for YAP1 and WWTR1.
  • CRISPR-based transcriptional modulation techniques.

Main Results:

  • Successful genetic knockout of YAP and TAZ genes was achieved.
  • Demonstrated feasibility of CRISPR for modulating YAP/TAZ expression.
  • Established methods for precise genomic engineering of Hippo pathway components.

Conclusions:

  • CRISPR offers powerful tools for dissecting YAP/TAZ function in Hippo signaling.
  • Genetic manipulation of YAP/TAZ using CRISPR opens new avenues for research.
  • This work provides a foundation for future therapeutic targeting of the Hippo pathway.