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Updated: Jan 31, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Synergistic coupling of in-line single-drop microextraction and on-line large-volume sample stacking for capillary
Jihye Kim1, Kihwan Choi2, Doo Soo Chung3
1Nuclear Chemistry Research Division, Korea Atomic Energy Research Institute, Daejeon, 34057, South Korea.
Abstract:
Single-drop microextraction (SDME) and large-volume sample stacking using an electroosmotic flow pump (LVSEP) were coupled with capillary electrophoresis/mass spectrometry (CE/MS) for sample cleanup and preconcentration. Without filtration or centrifugation of a soil sample containing debris, SDME using a pentanol acceptor drop was directly applied to the sample. After SDME, a large volume of the enriched pentanol extract was injected and further concentrated by LVSEP. For the drop formation in SDME and the sample matrix removal in LVSEP, a run buffer vial was temporarily placed to the electrospray tip, without any physical modification of the CE/MS interface. This method enabled the double preconcentration by SDME and LVSEP, achieving 600~1300-fold enrichments of anionic analytes including pesticide and herbicide compounds to provide limits of detection in the range of 0.4~0.8 ppb in soil. Graphical abstract ᅟ.
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