Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characteristics of GTP-mediated microsomal Ca2+ release.

S K Joseph1, H L Rice, C V Nicchitta

  • 1Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104.

Biochimica Et Biophysica Acta
|November 22, 1988
PubMed
Summary

Guanosine triphosphate (GTP) reversibly alters liver microsomal membrane permeability, affecting calcium (Ca2+) release and enhancing responses to inositol trisphosphate (IP3). This suggests GTP modulates a transmembrane pore, influencing intracellular Ca2+ signaling pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The impact of single versus double blastocyst transfer on pregnancy outcomes: A prospective, randomized control trial.

Facts, views & vision in ObGyn·2018
Same author

ENERGY METABOLISM DURING AIR EXPOSURE AND RECOVERY IN THE HIGH INTERTIDAL BIVALVE MOLLUSC GEUKENSIA DEMISSA GRANOSISSIMA AND THE SUBTIDAL BIVALVE MOLLUSC MODIOLUS SQUAMOSUS.

The Biological bulletin·2018
Same author

Single multivalent vaccination boosted by trickle larval infection confers protection against experimental lymphatic filariasis.

Vaccine·2013
Same author

Protective immune responses to biolistic DNA vaccination of Brugia malayi abundant larval transcript-2.

Vaccine·2012
Same author

IgG subclass responses to proinflammatory fraction of Brugia malayi in human filariasis.

The Indian journal of medical research·2012
Same author

Sensitization with anti-inflammatory BmAFI of Brugia malayi allows L3 development in the hostile peritoneal cavity of Mastomys coucha.

Acta tropica·2011

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Membrane Transport

Background:

  • Guanosine triphosphate (GTP) is known to release Ca2+ from liver microsomes.
  • Polyethylene glycol (PEG) enhances GTP-mediated Ca2+ release and responses to D-myo-inositol 1,4,5-trisphosphate (IP3).

Purpose of the Study:

  • To investigate the mechanism behind GTP-mediated Ca2+ release and its interaction with IP3 responses in liver microsomes.
  • To determine if GTP affects microsomal membrane permeability.

Main Methods:

  • Incubation of liver microsomes with GTP and PEG.
  • Use of GTP gamma S as a GTP antagonist.
  • Measurement of Ca2+ fluxes.
  • Assay of intraluminal esterase leakage.
  • Determination of mannose 6-phosphate hydrolysis latency.

Related Experiment Videos

  • Electron microscopy to assess vesicle fusion.
  • Microsomal density analysis.

    • Main Results:

    • GTP gamma S did not promote Ca2+ re-uptake after GTP-mediated release.
    • GTP's effects were reversible by washing or dilution.
    • PEG induced microsome aggregation, but not fusion.
    • GTP, in the presence of PEG, increased esterase leakage, reduced vesicle density, and decreased mannose 6-phosphate hydrolysis latency.
    • These permeability changes developed slowly, contrasting with rapid Ca2+ flux effects.
    • Mannose 6-phosphate permeability was restored upon GTP removal.

    Conclusions:

    • GTP induces non-specific permeability changes in microsomal membranes.
    • These permeability alterations may underlie GTP's effects on Ca2+ release.
    • GTP can reversibly modulate a transmembrane pore in microsomes, affecting ion and macromolecule passage.
    • This mechanism might link IP3-sensitive vesicles with other Ca2+-containing compartments.