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Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
Published on: June 16, 2023
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The long non-coding RNA-ROR promotes osteosarcoma progression by targeting miR-206
Dan Fei1, Guoqing Sui1, Yang Lu1
1Ultrasonographic Department, China-Japan Union Hospital of Jilin University, Changchun, P.R. China.
Journal of Cellular and Molecular Medicine
|December 20, 2018
Summary
Long intergenic non-protein coding RNA regulator of reprogramming (ROR) is upregulated in osteosarcoma (OS). ROR knockdown inhibits OS cell growth and metastasis, suggesting ROR is an oncogene and potential therapeutic target in OS.
Area of Science:
- Oncology
- Molecular Biology
- RNA Biology
Background:
- Long intergenic non-protein coding RNA regulator of reprogramming (lncRNA-ROR) is implicated in various cancers.
- The role of ROR in osteosarcoma (OS) initiation and progression is not well understood.
Purpose of the Study:
- To investigate the role of ROR in osteosarcoma.
- To explore the underlying molecular mechanisms of ROR in OS.
Main Methods:
- Quantitative real-time PCR to assess ROR expression in OS tissues.
- In vitro assays (proliferation, colony formation, migration, invasion) and in vivo tumor growth studies.
- Bioinformatics analysis, luciferase reporter assays, and miRNA inhibition experiments to elucidate the ROR-miRNA interaction.
Main Results:
- ROR expression was significantly elevated in OS tissues and correlated with advanced TNM stage, lymph node metastasis, and poor survival.
- ROR knockdown suppressed OS cell proliferation, migration, invasion, and tumor growth.
- ROR directly targets miR-206, and miR-206 inhibition partially reversed the effects of ROR knockdown.
Conclusions:
- ROR acts as an oncogene in osteosarcoma by sponging miR-206.
- ROR represents a potential therapeutic target for osteosarcoma patients.
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