Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conserved Binding Sites01:49

Conserved Binding Sites

5.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.2K
Conserved Binding Sites01:49

Conserved Binding Sites

1.9K
1.9K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.8K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.8K
Formation of Complex Ions03:45

Formation of Complex Ions

26.1K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.1K
Conservation of Energy in Control Volume01:14

Conservation of Energy in Control Volume

1.1K
Consider a turbine operating under steady-flow conditions. The control volume is drawn around the turbine, with fluid entering at one point and exiting at another. The turbine extracts energy from the fluid, which performs mechanical work (shaft work).
For steady flow systems, the time derivative of the stored energy becomes zero since there is no energy accumulation within the control volume. This simplifies the energy equation to:
1.1K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

14.4K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
14.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A DNA damage-activated kinase phosphorylates a transcriptional repressor to control bacterial immune pathway expression.

The EMBO journal·2026
Same author

Molecular mechanism of a single stranded DNA-stimulated bacterial immune peptidase.

bioRxiv : the preprint server for biology·2026
Same author

Phosphorylation remodels the mitotic centrosome matrix to generate bipartite γ-tubulin complex docking sites.

Science advances·2026
Same author

Do Caregiving Arrangements Influence Hospital Use Among Older Adults With Functional Limitations?

Health services research·2026
Same author

A chromatin-associated pool of Aurora A controls kinetochore-microtubule attachments to ensure chromosome biorientation.

Science advances·2026
Same author

Cancer type-specific variation in patterns of driver alterations across 50,000 tumors.

Cancer cell·2026

Related Experiment Video

Updated: Jan 31, 2026

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
07:59

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Published on: August 17, 2022

2.3K

The conserved XPF:ERCC1-like Zip2:Spo16 complex controls meiotic crossover formation through structure-specific DNA

Kanika Arora1, Kevin D Corbett1,2

  • 1Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA.

Nucleic Acids Research
|December 20, 2018
PubMed
Summary

Three meiosis proteins (Zip2, Zip4, Spo16) form a DNA-binding complex essential for crossover formation in yeast. This complex stabilizes early recombination intermediates, guiding gamete formation during meiosis.

More Related Videos

Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
06:38

Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes

Published on: November 22, 2017

12.8K
Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

10.1K

Related Experiment Videos

Last Updated: Jan 31, 2026

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
07:59

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Published on: August 17, 2022

2.3K
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
06:38

Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes

Published on: November 22, 2017

12.8K
Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

10.1K

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Eukaryotic meiosis requires inter-homolog crossovers for accurate chromosome segregation.
  • Meiosis-specific proteins (ZMMs) are crucial for crossover formation and spatial regulation.

Purpose of the Study:

  • Investigate the interaction and function of Saccharomyces cerevisiae ZMM proteins Zip2, Zip4, and Spo16.
  • Determine the structural basis for DNA binding and the role of the Zip2:Spo16 complex in meiotic recombination.

Main Methods:

  • Co-immunoprecipitation to assess protein interactions.
  • Crystal structure determination of the Zip2:Spo16 subcomplex.
  • DNA-binding assays using various DNA structures.

Main Results:

  • Zip2, Zip4, and Spo16 form a DNA-binding complex essential for crossover control.
  • The Zip2:Spo16 subcomplex structure is similar to XPF:ERCC1 but lacks endonuclease activity.
  • The complex binds specific DNA structures in early meiotic recombination intermediates.
  • Mutations in DNA-binding surfaces impair complex function, highlighting cooperative DNA binding.

Conclusions:

  • The Zip2:Zip4:Spo16 complex stabilizes early meiotic recombination intermediates.
  • This complex coordinates downstream factors for crossover formation and synaptonemal complex assembly.