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Ontogeny of calcium-binding proteins in the cingulate cortex of the guinea pig: The same onset but different

Beata Hermanowicz-Sobieraj1, Krystyna Bogus-Nowakowska1, Maciej Równiak1

  • 1Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Pl. Łódzki 3, 10-727 Olsztyn, Poland.

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
|December 20, 2018
PubMed

Insights

This study reveals distinct developmental timelines for calcium-binding proteins (CaBPs) in the guinea pig cingulate cortex (CC). Calbindin D28k (CB) and calretinin (CR) peak prenatally, while parvalbumin (PV) increases postnatally, correlating with cortical maturation.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Neuroanatomy

Background:

  • The cingulate cortex (CC) plays a crucial role in cognitive functions.
  • Understanding the developmental trajectory of neuronal populations expressing calcium-binding proteins (CaBPs) is essential for comprehending CC maturation.
  • Calcium-binding proteins, including calbindin D28k (CB), calretinin (CR), and parvalbumin (PV), are key markers for distinct neuronal subtypes.

Purpose of the Study:

  • To compare the density and distribution of CB, CR, and PV-containing neurons during prenatal, newborn, and postnatal development in the guinea pig CC.
  • To investigate the co-distribution patterns of these CaBPs throughout CC ontogeny.
  • To correlate changes in CaBP-positive neuron density with known developmental milestones in the guinea pig.

Main Methods:

  • Immunohistochemical analysis of CB, CR, and PV expression in the guinea pig cingulate cortex.
  • Quantitative assessment of neuronal density for each CaBP at different developmental stages (prenatal, newborn, postnatal).
  • Examination of the spatial distribution and co-localization of neurons expressing different CaBPs.

Main Results:

  • CB-positive neurons showed the highest overall density in the developing CC.
  • CB and CR immunoreactivity peaked during the prenatal period, indicating early developmental roles.
  • PV-positive neuron density increased significantly in the postnatal period, suggesting a role in later maturation of inhibitory pathways.

Conclusions:

  • The developmental timing of CB, CR, and PV expression in the guinea pig CC differs, with CB and CR being prominent prenatally and PV postnatally.
  • These distinct expression patterns coincide with critical developmental events, such as eye opening and the maturation of cortical inhibitory circuits.
  • The findings provide insights into the differential roles of CaBP-expressing interneurons in the ontogeny of the cingulate cortex.

Keywords:
CalbindinCalretininCingulate cortexGuinea pigOntogenyParvalbumin

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