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Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
Published on: May 9, 2017
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Comparative transcriptomic analysis of bovine papillomatosis
Débora M Barreto1, Gerlane S Barros1, Lucas A B O Santos1
1Laboratory of Molecular Genetics and Biotechnology, Department of Biology, Center for Biological and Health Sciences, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
BMC Genomics
|December 21, 2018
Summary
Bovine papillomavirus (BPV) infection alters host gene expression, with 1343 genes differentially regulated. This study identifies potential markers for controlling BPV-induced lesions and interrupting viral infection.
Area of Science:
- Veterinary Virology
- Molecular Biology
- Transcriptomics
Background:
- Bovine papillomavirus (BPV) causes hyperproliferative lesions in cattle.
- BPV alters host cell cycle gene regulation, leading to lesion formation.
- The specific host genes regulated by BPV infection remain largely unknown.
Purpose of the Study:
- To identify differentially expressed genes in BPV-infected cattle using next-generation RNA sequencing.
- To elucidate host gene regulation mechanisms involved in BPV-induced lesion development.
- To discover potential marker genes for BPV disease control.
Main Methods:
- Next-generation RNA sequencing (RNA-Seq) was employed for transcriptome analysis.
- Gene expression profiles of BPV-infected and non-infected cattle were compared.
- Statistical analysis (FDR < 0.05) was used to identify differentially expressed genes.
Main Results:
- A total of 1343 genes exhibited differential expression (FDR < 0.05).
- 655 genes were significantly upregulated, and 688 genes were significantly downregulated in infected animals.
- The majority of differentially expressed genes were linked to BPV infection pathways.
Conclusions:
- This study provides a large-scale evaluation of gene expression changes associated with BPV infection.
- Identified host genes and pathways offer insights into lesion development.
- Novel targets for interrupting the BPV infection cycle may be discovered.
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