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Efficient in vitro expression of interferon alpha analogs using SP6 polymerase and rabbit reticulocyte lysate
1Centre for Molecular Biology and Medicine, Monash University, Clayton, Victoria, Australia.
Gene Analysis Techniques
|January 1, 1988
Abstract:
We have investigated the use of in vitro expression as a quick and convenient means of screening large numbers of interferon (IFN) analogs generated using in vitro mutagenesis. The IFN-alpha 1 mRNA generated from DNA template using SP6 RNA polymerase is efficiently translated in rabbit reticulocyte lysate (RRL). The antiviral specific activity of this RRL-synthesized IFN-alpha l is equivalent to the yeast-synthesized protein. In contrast with the yeast-expression system, where some IFN-alpha analogs are poorly expressed, all analogs tested were well expressed in RRL.