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High-frequency transformation of Rhizobium meliloti
J Courtois1, B Courtois, J Guillaume
1Laboratoire de Microbiologie, U.F.R. de Biologie, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Journal of Bacteriology
|December 1, 1988
Abstract:
Transformation of R factor RP4 and its derivative pRK290 from Escherichia coli to Rhizobium meliloti is reported. The efficiency of transformation was in the range of 10(-5) per viable cell. In addition, chromosomal DNA prepared from one R. meliloti strain resistant to streptomycin was transferred to the isoleucine-valine-requiring mutant susceptible to streptomycin.
