Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid method for fetal brain fixation.

J M Nicholls1

  • 1Department of Histopathology, Queen Elizabeth Hospital, Woodville, South Australia.

Journal of Clinical Pathology
|September 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tropism of the novel human betacoronavirus lineage C virus in human ex vivo and in vitro cultures: potential transmissibility and pathogenesis in humans (abridged secondary publication).

Hong Kong medical journal = Xianggang yi xue za zhi·2021
Same author

SARS-CoV-2 infection in conjunctival tissue - Authors' reply.

The Lancet. Respiratory medicine·2020
Same author

Characterization of a novel coronavirus responsible for severe acute respiratory syndrome.

International congress series·2020
Same author

Tropism of influenza B viruses in human respiratory tract explants and airway organoids.

The European respiratory journal·2019
Same author

Prevention and treatment of swine-origin influenza virus with interferon: an in vivo and ex vivo study.

Hong Kong medical journal = Xianggang yi xue za zhi·2018
Same author

Tumor suppressor Alpha B-crystallin (CRYAB) associates with the cadherin/catenin adherens junction and impairs NPC progression-associated properties.

Oncogene·2011

Researchers developed a faster method for fixing fetal brains, preserving both gross and microscopic structures. This technique offers excellent tissue preservation with minimal cost increase over standard formalin fixation.

Area of Science:

  • Neuroscience
  • Histology
  • Pathology

Background:

  • Standard brain tissue fixation is time-consuming.
  • A need exists for quicker, effective fixation methods in research and diagnostics.

Purpose of the Study:

  • To develop and evaluate a faster alternative to standard brain tissue fixation.
  • To assess the preservation quality of the proposed method.

Main Methods:

  • Whole fetal brains were fixed in situ using a mercuric-based fixative.
  • The subarachnoid space was perfused with Heidenhain's Susa fixative overnight.
  • Brains were removed, stored in Susa's fixative for three days, then sliced and processed.

Main Results:

  • The method achieved excellent preservation of gross brain architecture.

Related Experiment Videos

  • Microscopic architecture was also excellently preserved.
  • The overall cost was only marginally higher than commercial formalin.

    • Conclusions:

    • This in situ perfusion fixation method offers a quicker alternative to standard brain tissue processing.
    • The technique provides high-quality preservation suitable for histological analysis.
    • The cost-effectiveness makes it a viable option for laboratories.