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Updated: Jan 31, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
RXXPEG motif of MERIT40 is required to maintain spindle structure and function through its interaction with
Duo Zheng1,2, Wangqing Xie1,2, Li Li3
1Shenzhen Longhua District Central Hospital, Shenzhen, 518110, China.
Abstract:
Deubiquitinase BRISC complex plays important role in the maintenance of spindle structure and function; however, the underlying mechanism remains largely undefined. Here we demonstrated that MERIT40, a core component of BRISC complex, directly interacts with the RXXPEG motif in the ARC-V domain of Tankyrase1(TNKS1). Mutation of the RXXPEG motif in the MERIT40 (R28A) disrupted its interaction with TNKS1. Consistent with these data, R28A mutant cells displayed multiple mitotic defects including aberrant spindle assembly and chromosome misalignment. These results support a critical role of RXXPEG motif of MERIT40 in BRISC-mediated regulation of TNKS1 function during spindle assembly.
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