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Updated: Jan 31, 2026
08:30
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
Published on: August 21, 2016
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Profiling of lymphoma from formalin-fixed paraffin-embedded tissue
Ryan S Robetorye1, Alanna Maguire2, Alison C Rosenthal3
1Department of Laboratory Medicine and Pathology, Mayo Clinic Arizona, Phoenix, AZ.
Seminars in Hematology
|December 22, 2018
Summary
Molecular profiling advances lymphoma understanding, but FFPE tissues pose challenges for diagnostics. This study explores profiling techniques and FFPE applications in lymphoma molecular testing.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- Molecular profiling of lymphoma has significantly advanced disease biology understanding, leading to refined diagnostic categories and identification of subtypes, immune responses, and prognostic groups.
- Discoveries in lymphoma molecular profiling have predominantly utilized unfixed, snap-frozen samples.
- Clinical biopsy materials are typically formalin-fixed and paraffin-embedded (FFPE) tissues, presenting unique challenges for molecular analysis.
Purpose of the Study:
- To discuss the impact of molecular profiling on lymphoma research and diagnostics.
- To highlight the challenges of utilizing FFPE tissues for molecular diagnostic testing in lymphoma.
- To review various molecular profiling techniques and present a clinical application example using FFPE lymphoma tissues.
Main Methods:
- Review of existing literature on molecular profiling in lymphoma.
- Analysis of challenges associated with FFPE tissue processing for molecular diagnostics.
- Description of various molecular profiling techniques applicable to lymphoma.
- Case study illustrating the clinical application of molecular profiling on FFPE lymphoma samples.
Main Results:
- Molecular profiling has revolutionized lymphoma classification and understanding of disease heterogeneity.
- FFPE tissues present technical hurdles for molecular profiling due to fixation and embedding processes.
- Various molecular profiling techniques demonstrate potential for clinical application with FFPE samples.
- Successful clinical application of molecular profiling on FFPE lymphoma tissues has been demonstrated.
Conclusions:
- Molecular profiling is crucial for advancing lymphoma diagnosis and understanding.
- Overcoming FFPE tissue challenges is essential for widespread clinical molecular diagnostics in lymphoma.
- The integration of molecular profiling techniques with FFPE tissues holds significant promise for personalized lymphoma management.
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