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Published on: January 17, 2025
Voltammetric glucose biosensor based on glucose oxidase encapsulation in a chitosan-kappa-carrageenan polyelectrolyte
Ilhem Rassas1, Mohamed Braiek2, Anne Bonhomme2
1University of Lyon, Institute of Analytical Sciences, Villeurbanne, France; University of Monastir, Faculty of Sciences of Monastir, Laboratory of Advanced Materials and Interfaces, Monastir, Tunisia.
Abstract:
In this work, a new design of voltammetric glucose biosensor, based on the encapsulation of glucose oxidase (GOx) in a chitosan/κ‑carrageenan (CHIT/CAR) polyelectrolyte complex (PEC) using a simple coacervation process is presented. A conductometric monitoring of this is performed. Spectroscopic and morphological characterization of the PEC film encapsulating GOx is carried out. Compared to biosensors based on a chitosan film, a more sensitive voltammetric detection of glucose is obtained. Using square wave voltammetry (SWV), the CHIT/CAR PEC based biosensor exhibits a wide linearity range from 5 μM to 7 mM glucose with a detection limit of 5 μM. Excellent selectivity against ascorbic acid, uric acid and urea is observed and the applicability of the biosensor for glucose detection in spiked saliva samples was demonstrated.
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