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Updated: Jan 31, 2026

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
Published on: November 29, 2024
Reliability of human cortical organoid generation
Se-Jin Yoon1, Lubayna S Elahi1, Anca M Pașca2
1Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA.
Abstract:
The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.
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