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Updated: Jan 31, 2026

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
Published on: July 5, 2021
Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces
Sinem Yeniyol1, John Lawrence Ricci2
1Department of Oral Implantology, İstanbul University, Faculty of Dentistry, İstanbul, Turkey.
Purpose:
This study aimed to evaluate the initial adhesion morphology and alkaline phosphatase (ALP) activity of murine pre-osteoblastic MC3T3-E1 cells cultured on anatase/rutile mixed-phase TiO2 thin films with photocatalytical activity with previously confirmed antibacterial properties.
Materials And Methods:
Anatase/rutile mixed-phase TiO2 thin films fabricated by anodization and annealing of cpTi were used to culture MC3T3-E1 cells to evaluate the initial cellular adhesion morphology and ALP activity in vitro.
Results:
Compared with MC3T3-E1 cells cultured on cpTi substrates and the control group, cells cultured on anatase/rutile mixed-phase TiO2 thin films exhibited similar ALP levels after cell culture day 9.
Conclusion:
Anodizing and annealing processes fabricate multifunctional surfaces on cpTi with improved osteogenic properties for implants.
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