Related Experiment Video
Updated: Jan 31, 2026

Induction of Hypoxia in Living Frog and Zebrafish Embryos
Published on: June 26, 2017
Development and characterization of 33 microsatellite loci for the tiger frog Hoplobatrachus rugulosus (Wiegmann
Zhiqiang Chen1, Guohua Ding, Yanmei Wang
1College of Ecology, Lishui University, Lishui 323000, Zhejiang, People's Republic of China. guwoding@qq.com.
Abstract:
The tiger frog Hoplobatrachus rugulosus (Wiegmann 1834) is a large robust dicroglossid frog widely distributed in southern China, Malaysia, Myanmar, Vietnam and Thailand. The escaped bred tiger frog introduced from Thailand hybridized with Chinese native population may have affected the genetic diversity of local Chinese tiger frogs. However, previous microsatellite loci of this species do not offer enough information to construct the genetic map. Here, we reported 33 new microsatellite loci from transcriptome sequencing for H. rugulosus. Alleles ranged between 1 and 10 per locus and only one locus (HRT001) was monomorphic. The polymorphic information content, observed and expected heterozygosity were 0-0.794, 0-0.969 and 0-0.831, respectively. None of the loci was observed in linkage disequilibrium and two loci (HRT023 and HRT068) deviated from Hardy-Weinberg equilibrium after Bonferroni correction for multiple tests. These transcriptome-derived microsatellite markers will be usedto study the genetic divergence and construct the genetic map in H. rugulosus.
Related Concept Videos
Root Loci for Positive-Feedback Systems
The construction rules for the root locus in positive feedback systems are similar to those in...
Design Example: Frog Muscle Response
When the switch connecting the RL circuit is closed, a brief muscle contraction is observed. This is because, at a steady state, the inductor acts like a short...
Cis-regulatory Sequences
Sequences
Sanger Sequencing
Arithmetic Sequences

