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Updated: Jan 31, 2026

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
Development of a peptide-based fluorescent probe for biological heme monitoring
Laura D Newton1, Sofia I Pascu, Rex M Tyrrell
1Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, UK. ie203@bath.ac.uk.
Abstract:
Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation.
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