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Exploring neuropeptide signalling through proteomics and peptidomics.

Samantha Louise Edwards1, Lucas Mergan1, Bhavesh Parmar1

  • 1a Animal Physiology and Neurobiology, Department of Biology , KU Leuven , Leuven , Belgium.

Expert Review of Proteomics
|December 22, 2018
PubMed
Summary

Neuropeptide signaling is vital for organismal responses. Proteomics and peptidomics offer powerful tools to understand neuropeptide processing and function in endocrine pathways.

Keywords:
Mass spectrometryneuropeptide maturationneuropeptide signallingneuropeptidesneuroproteomicspeptidomics

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Area of Science:

  • Neuroendocrinology
  • Molecular Biology
  • Biochemistry

Background:

  • Neuropeptides act as critical signaling molecules (neurotransmitters, neuromodulators, neurohormones) in multicellular organisms.
  • Understanding neuropeptide integration in endocrine pathways is key to deciphering organismal responses to internal and external cues.

Purpose of the Study:

  • This review focuses on proteomics and peptidomics approaches for studying neuropeptide signaling systems.
  • It highlights key breakthroughs and methodologies in the field.

Main Methods:

  • Differential mass spectrometry and reverse genetic approaches are commonly employed.
  • Advanced liquid chromatography coupled with mass spectrometry has become central to proteomics.
  • Peptidomics offers distinct advantages over standard proteomics for studying neuropeptide processing.

Main Results:

  • Proteomics reveals neuropeptide maturation and identifies downstream signaling targets.
  • Peptidomics facilitates the study of neuropeptide processing and signal pathway identification.
  • Advancements in isolation techniques and equipment sensitivity enhance the elucidation of endocrine signaling.

Conclusions:

  • Proteomics and peptidomics are increasingly vital for understanding the functional implications of endocrine signaling.
  • Future technical progress, particularly in single-neuron peptidomic profiling, promises a comprehensive understanding of endocrine signaling.