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Updated: Jan 31, 2026

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
Ideal-Filter Capillary Electrophoresis (IFCE) Facilitates the One-Step Selection of Aptamers
An T H Le1, Svetlana M Krylova1, Mirzo Kanoatov1
1Centre for Research on Biomolecular Interactions, York University, Toronto, Ontario, M3J 1P3, Canada.
Abstract:
Selection of aptamers from oligonucleotide libraries currently requires multiple rounds of alternating steps of partitioning of binders from nonbinders and enzymatic amplification of all collected oligonucleotides. Herein, we report a highly practical solution for reliable one-step selection of aptamers. We introduce partitioning by ideal-filter capillary electrophoresis (IFCE) in which binders and nonbinders move in the opposite directions. The efficiency of IFCE-based partitioning reaches 109 , which is ten million times higher than that of typical solid-phase partitioning methods. One step of IFCE-based partitioning is sufficient for the selection of a high-affinity aptamer pool for a protein target. Partitioning by IFCE promises to become an indispensable tool for fast and robust selection of binders from different types of oligonucleotide libraries.
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