Dried blood spots for reliable biomonitoring of poly- and perfluoroalkyl substances (PFASs)
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Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Published on: November 8, 2015
Somrutai Poothong1, Eleni Papadopoulou2, Elsa Lundanes3
1Department of Environmental Exposure and Epidemiology, Norwegian Institute of Public Health, P.O. Box 4404, Nydalen, NO-0403 Oslo, Norway; Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway.
Abstract:
Dried blood spot (DBS) sampling has gained attention in several scientific areas because of the low sampling burden. The study aimed to develop a method for the determination of poly- and perfluoroalkyl substances (PFASs) in DBS using a standardized blood volume. The DBS method using a simple methanol extraction followed by online solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry quantification was validated. Only 30 μL of blood is required. Based on the measurements of DBS dispersed areas from known blood volumes (20-70 μL), the blood volume on a 3 mm diameter DBS subsample was calculated to be 3.3 μL (median, n = 708 measurements, 59 adults). Strong correlations of PFAS concentrations between finger prick DBSs and venous whole blood samples (n = 57) were found (rho 0.72-0.97, p < 0.0001). Also, Passing-Bablok regressions and Bland-Altman plots demonstrated good agreements of PFAS concentrations in finger prick DBSs and venous whole blood samples. This finding indicates that the DBS method was satisfactory, and allows straightforward analysis of PFASs in DBS without hematocrit correction. This DBS method is reliable for accurate determination of PFASs and has a high potential for use of self-collected DBS in large-scale biomonitoring studies as well as for archived DBS samples.
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