Interaction of statins with phospholipid bilayers studied by solid-state NMR spectroscopy
Leisan F Galiullina1, Holger A Scheidt2, Daniel Huster2
1Kazan (Volga Region) Federal University, 18 Kremlevskaya St., 420008 Kazan, Russian Federation.
Biochimica Et Biophysica Acta. Biomembranes
|December 23, 2018
Summary
Statins interact with cell membranes, influencing their structure and dynamics. These interactions vary between different statins, affecting their overall pharmacological actions beyond cholesterol reduction.
Area of Science:
- Biochemistry
- Biophysics
- Pharmacology
Background:
- Statins inhibit HMG-CoA reductase, lowering LDL cholesterol and reducing atherosclerosis risk.
- Intermolecular differences among statins suggest broader pharmacologic actions beyond HMG-CoA reductase inhibition.
- Cellular membrane localization is hypothesized to influence statin biological effects.
Purpose of the Study:
- To investigate the interactions of various statins with phospholipid membranes.
- To examine the influence of statins on membrane structure and dynamics.
Main Methods:
- Solid-state Nuclear Magnetic Resonance (NMR) techniques, specifically 1H NOESY MAS NMR.
- Quantitative determination of statin distribution within lipid bilayers using cross-relaxation rates.
Main Results:
- Atorvastatin, cerivastatin, fluvastatin, rosuvastatin, and pravastatin intercalate the lipid-water interface of POPC membranes to varying degrees.
- Lovastatin exhibited minimal impact on lipid packing and chain order.
- Pravastatin significantly reduced lipid chain order, while other statins slightly decreased it in the middle segments of phospholipid chains.
Conclusions:
- Statin interactions with phospholipid membranes differ significantly.
- These membrane interactions contribute to the diverse pharmacological profiles of statins.
- Understanding statin-membrane dynamics provides insights into their broader therapeutic effects.
Keywords:
(1)H NMRIntermolecular cross-relaxationNuclear Overhauser enhancement spectroscopyPhospholipid membranesStatinsMore Related Videos
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