GABA-mediated control of transient signals in the inner retina

The effects of chemicals on visual activity was investigated by recording the proximal negative response (PNR) in the perfused eyecup of frogs. Various amino acids reversibly decrease or abolish the PNR over an equivalent concentration range but differ in this depressive action in the presence of convulsant alkaloids. Depression produced by GABA was antagonized by picrotoxin or bicuculline; while glycine-produced depression was selectively antagonized by strychnine. In addition to selective antagonism of amino acids, the convulsants produced differential enhancement of sustained and transient phases of the PNR. In this respect, the effects of picrotoxin and bicuculline are similar to those of semicarbazide and chloride-free solutions which may also inactivate GABA pathways. The effects of these chemicals on the time course and input-output relation of the PNR suggest that processes involving GABA may regulate the transformation from sustained to transient signals which occurs in the inner retina.