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Updated: Jan 31, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
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Label-Free High-Throughput Leukemia Detection by Holographic Microscopy

Matthias Ugele1,2, Markus Weniger1, Manfred Stanzel1

  • 1In-Vitro DX and Bioscience Department of Strategy and Innovation Siemens Healthcare GmbH Günther-Scharowsky-Str. 1 91058 Erlangen Germany.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|December 25, 2018
PubMed

Complete blood count and differentiation of leukocytes (DIFF) belong to the most frequently performed laboratory diagnostic tests. Here, a flow cytometry-based method for label-free DIFF of untouched leukocytes by digital holographic microscopy on the rich phase contrast of peripheral leukocyte images, using highly controlled 2D hydrodynamic focusing conditions is reported. Principal component analysis of morphological characteristics of the reconstructed images allows classification of nine leukocyte types, in addition to different types of leukemia and demonstrates disappearance of acute myeloid leukemia cells in remission. To exclude confounding effects, the classification strategy is tested by the analysis of 20 blinded clinical samples. Here, 70% of the specimens are correctly classified with further 20% classifications close to a correct diagnosis. Taken together, the findings indicate a broad clinical applicability of the cytometry method for automated and reagent-free diagnosis of hematological disorders.

Keywords:
digital holographic microscopylabel‐free detectionleukemiamicrofluidics

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