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Updated: Jan 31, 2026

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
Published on: February 2, 2018
Computational and experimental data on electrostatic density and stacking tendency of asymmetric cyanine 5 dyes
S J Spa1,2, A W Hensbergen1, S van der Wal1
1Interventional Molecular Imaging Laboratory, Department of Radiology, Leiden University Medical Center, the Netherlands.
Abstract:
Far-red dyes such as cyanine 5 (Cy5) are gaining interest in (bio)medical diagnostics as they have promising features in terms of stability and brightness. Here, the electrostatic density and stacking tendency in different solvents of nine systematically altered asymmetrical Cy5 dyes are reported. In addition to this, the influence of molecular alterations on the vibronic coupling was reported. The data presented supplement to the recent study "The influence of systematic structure alterations on the photophysical properties and conjugation characteristics of asymmetric cyanine 5 dyes" (Spa et al., 2018).
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