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Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Escherichia coli O157:H7 transcriptome datasets for comparison of RNA-seq and microarray platforms
Ewa Grabowiecka1, David Martin1, Louise Crozier2
1The University of Dundee, Dundee, UK.
Abstract:
Whole transcriptome analysis to investigate differential gene expression and regulatory adaption can be carried out on two different technological platforms: by probe hybridisation to microarrays or by RNAseq for deep sequencing. Since there are difference in terms of their genome coverage, sensitivity and cost, there is a requirement for robust comparisons to determine the platform of choice. Here, we present datasets for the whole transcriptional response verocytoxigenic Escherichia coli (VTEC) obtained from RNA-seq and microarray platforms in response to spinach, together with a comparison between the datasets (available at Array Express: E-MTAB-3249, E-MTAB-4120, E-MTAB-7441).
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