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Related Concept Videos

Curing of Concrete01:20

Curing of Concrete

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The hydration of cement takes place within the water-filled capillary pores. However, environmental elements can disrupt this process by evaporating water from the concrete surfaces. Sealed concrete with a water-cement ratio below 0.5 experiences self-desiccation, leading to water loss. The water loss in concrete is mitigated by curing. This technique involves keeping the concrete saturated to maintain the necessary temperature and moisture conditions, to optimally fill the spaces in the cement...
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Curing Methods01:26

Curing Methods

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Concrete members with a small surface-to-volume ratio are cured by oiling and moistening the forms before casting the concrete member. These forms can be left in place for a prolonged period to prevent moisture loss, and can be wetted if made of a material suitable for wetting. If the forms are removed early, the concrete member is moistened and covered with polythene sheets to maintain moisture. For large horizontal concrete surfaces exposed to dry weather, a temporary covering is suspended...
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Accelerated Curing of Concrete01:25

Accelerated Curing of Concrete

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Accelerating concrete curing is achieved by applying heat and additional moisture. This process accelerates the hydration of the cement, resulting in an earlier strength gain in the concrete. Steam curing is a method wherein the concrete products are either transported through a chamber on a conveyor belt or encased in plastic, allowing steam at atmospheric pressure to circulate freely around them. This process begins with a phase of moist curing that typically lasts between 3 to 5 hours, after...
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Components of Stress01:23

Components of Stress

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Stress analysis under multiple loading conditions is intricate, necessitating a comprehensive grasp of normal and shearing stresses. Consider a small cube at point O, subjected to stress on all six faces, visible or not. Normal stress components σx, σy, σz act perpendicularly to the x, y, and z axes. Shearing stress components τxy and τxz are exerted on faces perpendicular to these axes.
Interestingly, the hidden cube faces also experience these stresses, equal and...
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Components of Language01:24

Components of Language

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Language, whether spoken, signed, or written, consists of specific components: lexicon and grammar. The lexicon is the vocabulary of a language, comprising its words. Grammar is the set of rules used to convey meaning through the lexicon. For example, English grammar adds “-ed” to most verbs to indicate past tense. Words are formed by combining phonemes, which are the basic sound units of a language. Different languages have different sets of phonemes (e.g., “ah” vs.
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Fixing Double-strand Breaks02:04

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The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
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Evaluation of the Curing of Adhesive Systems by Rheological and Thermal Testing
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[Development of double-component rapid curing bioadhesive].

Juan Huang1, Haorong Li2, Qian Wang3

  • 1College of Life Sciences, Zhejiang Sci-Tec University, Hangzhou 310018, P.R.China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
|December 25, 2018
PubMed
Summary
This summary is machine-generated.

Mussel foot proteins (MFp) were modified to create a strong, fast-curing bioglue. This new protein-based adhesive shows superior performance compared to commercial tissue adhesives.

Keywords:
adhesive proteinhyaluronic acidlayer-by-layer assemblymussel foot proteinrapid solidification

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Area of Science:

  • Biomaterials Science
  • Protein Engineering
  • Adhesive Technology

Context:

  • Mussel foot proteins (MFp) are known for their underwater adhesion capabilities.
  • Developing biocompatible bioglues with enhanced adhesive strength and rapid curing is a significant challenge.
  • Existing protein-based adhesives often suffer from low adhesion and slow curing times.

Purpose:

  • To engineer a soluble recombinant SUMO-MFp fusion protein (SFp3) for enhanced adhesive properties.
  • To improve the adhesion and curing speed of MFp-based bioglues.
  • To provide a solution for the limitations of current protein-based adhesives.

Summary:

  • Recombinant SUMO-MFp fusion protein (SFp3) was expressed in E. coli and modified with DOPA using mushroom tyrosinase.
  • The DOPA-containing SFp3 (DSFp3) mixed with hyaluronic acid demonstrated over twice the adhesion strength of Dermabond® and reached 52% strength in 5 minutes on cowhide.
  • Layer-by-layer assembly of hyaluronic acid with DSFp3 formed compact structures, confirmed by biofilm interferometry and SEM.

Impact:

  • This research offers a promising biocompatible bioglue with significantly improved adhesion strength and curing speed.
  • The findings provide a theoretical basis for overcoming the limitations of protein-based bioglues.
  • The developed bioglue has broad application prospects in tissue repair and biomedical applications.