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Updated: Jan 31, 2026

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
Estimated dietary intake of nitrate and nitrite from meat consumed in Fiji
Adrian A Chetty1, Surendra Prasad2, Olívia Castro Pinho3
1School of Biological and Chemical Sciences, Faculty of Science, Technology and Environment, The University of the South Pacific, Private Mail Bag, Suva, Fiji; School of Sciences, College of Engineering, Science and Technology, Fiji National University, Nasinu, Fiji; Faculty of Nutrition and Food Sciences, University of Porto, R. Dr. Roberto Frias S/N, 4200-465 Porto, Portugal.
Abstract:
To assess the risk posed by meat consumption to the Fiji and Pacific populace, the present study reports nitrate and nitrite in meat. Twelve commercially available meat products, with a total of 210 fresh and processed meat samples, were analysed for nitrate and nitrite by an optimised RP-HPLC technique with isocratic elution using n-octylamine in 20.0% methanol at pH 6.60. The nitrate content in the meat samples ranged from 0.00 to 124 mg kg-1 whereas the nitrite ranged from 0.00 to 164 mg kg-1. The study shows that the nitrate and nitrite contents of meat samples in Fiji were below the maximum level proposed by European Union legislation but above the limit set by Food Standards Australia and New Zealand (FSANZ). The estimated dietary intake of nitrate and nitrite was calculated from a 24-h diet recall study as well as from Fiji's food balance sheets.
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