Related Experiment Video
Updated: Jan 31, 2026

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
Published on: July 7, 2012
Effects of Growth Regulators and Gelling Agents on Ex Vitro Rooting of Raspberry
Vadim Lebedev1,2, Mikhail Arkaev3, Mariya Dremova4,5
1Pushchino State Institute of Natural Sciences, Prospekt Nauki 3, Pushchino, Moscow Region 142290, Russia. vglebedev@mail.ru.
Abstract:
Successful acclimatization and ex vitro rooting are among the key factors reducing the cost of micropropagated plants. We compared the survival of seven Russian cultivars of raspberry (Rubus idaeus) after rooting in vitro and ex vitro. Rooted shoots adapted to nonsterile conditions much better than nonrooted ones, with survival rates of 81%⁻98% versus 43%⁻76%, respectively. We studied the effects of different combinations of plant-growth regulators and gelling agents added to a proliferation medium on ex vitro rooting of primocane-fruiting raspberry cultivar "Atlant". Reducing the agar concentration from 8 to 6.5 g/L increased the multiplication rate, but caused shoot hyperhydricity. The highest survival rate (97.2%) was observed for shoots grown in a medium containing 0.2 and 0.1 mg/L IBA, and gelled with 5 g/L agar and 0.2 g/L Phytagel. The microshoot height at the multiplication stage did not correlate with the plant growth during acclimatization. The obtained results can be used in the commercial micropropagation of the raspberry.
Related Concept Videos
Primary and Secondary Growth in Roots and Shoots
Meristems and Plant Growth
Epigenetic Regulation
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
GTPases and their Regulation
Large G-proteins,...
Regulated Protein Degradation
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

