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Related Concept Videos

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Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
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In vitro dissolution and drug release tests assess how quickly and how much of a drug is released from its dosage form into an aqueous medium under standardized laboratory conditions. These tests are essential tools in pharmaceutical development and quality assurance, offering insight into the drug's performance before clinical use.During formulation development, dissolution testing identifies incomplete or inconsistent drug release issues. It also supports decisions on selecting the optimal...
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The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
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Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
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Enabling drug discovery and development through single-cell imaging.

Andrea K Pomerantz1, Farid Sari-Sarraf1, Kerri J Grove2

  • 1a Analytical Sciences & Imaging , Novartis Institutes for BioMedical Research Inc ., Cambridge , MA , USA.

Expert Opinion on Drug Discovery
|December 26, 2018
PubMed
Summary
This summary is machine-generated.

Single-cell imaging assays enhance drug discovery by analyzing rare cells and cellular heterogeneity. Technologies like microfluidics, mass spectrometry, and imaging mass cytometry (IMC) offer scalable, multiplexed insights for developing safer therapies.

Keywords:
BioinformaticsCAR-Tfluorescence microscopyimage analysisimaging mass cytometryimaging mass spectrometryimmuno-oncologymicrofluidicsmultiplexingoncologytissue

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Area of Science:

  • Biotechnology
  • Drug Discovery
  • Cellular Analysis

Background:

  • Single-cell imaging assays are increasingly vital in drug discovery.
  • These assays enable interrogation of rare cell subpopulations with high sensitivity and multiplexing.
  • Technological advancements have significantly boosted the throughput and capabilities of these methods.

Purpose of the Study:

  • To review key single-cell imaging technologies used in drug discovery.
  • To highlight the utility of these methods in understanding cellular heterogeneity and target engagement.
  • To discuss the future potential and challenges of single-cell imaging in therapeutic development.

Main Methods:

  • Single-cell microscopy combined with microfluidics.
  • Mass spectrometric imaging for subcellular compound localization.
  • Imaging mass cytometry (IMC) for high-parameter single-cell analysis.

Main Results:

  • These technologies offer scalability and multiplexing for detailed cellular analysis.
  • They provide definitive data on cellular heterogeneity and target engagement.
  • Examples demonstrate their utility in advancing drug discovery pipelines.

Conclusions:

  • Single-cell analysis is crucial for developing safer, more effective therapies, especially for novel modalities like CAR-T and gene editing.
  • Adoption requires investment in advanced computational and bioinformatic tools to handle complex imaging data.
  • These approaches promise to revolutionize personalized medicine and targeted drug development.