Related Experiment Video
Updated: Jan 31, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
Published on: June 23, 2022
Comparing bacterial, fungal, and human cell concentrations with rapid adenosine triphosphate measurements for
Sarah E Kwan1, Jordan Peccia1, Jonathan Simonds1
1Department of Chemical and Environmental Engineering, Yale University, New Haven, CT.
Background:
The goal of this study was to test for associations between adenosine triphosphate (ATP) content and microbial concentrations on desk surfaces in school classrooms.
Methods:
ATP bioluminescence and quantitative polymerase chain reaction (qPCR) techniques were employed to measure total bacterial, fungal, and human cell concentrations on 66 high-traffic desks spread across 9 schools: 3 in Connecticut (CT) and 6 in Oklahoma (OK). In CT, 6 samples were taken from each desk, 1 precleaning and 5 postcleaning (after 30 minutes, 1 day, 3 days, 7 days, and 21 days). In OK, samples were taken immediately before and after cleaning each desk.
Results:
Based on simple linear regression analyses, ATP values were good predictors of microbial concentrations (r = 0.8, P = .003) in both CT school postcleaning samples and OK pre- and postcleaning samples (r = 0.7, P = .00002). However, biomass reductions measured after cleaning were 1.5-2 times greater when measured by ATP than by qPCR (P = .007).
Conclusions:
Overall, ATP bioluminescence measurements correlate with qPCR-based surface measurements on school desks but may overestimate the physical removal of bacteria and fungi due to cleaning.
Related Concept Videos
Concentration Cells
Consider the following voltaic cell:
Drug Concentrations: Measurements
Plasma...
Microbial Growth Measurement: Direct Methods
Microbial Growth Measurement: Indirect Methods
Contaminants and Errors
Another key consideration is determining the appropriate number of samples required to...
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

