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Updated: Jan 31, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Viability of Chlorine-injured Escherichia Coli O157:H7 on Fresh-cut Cabbage during Cold Storage in High CO2
1Faculty of Biology-Oriented Science and Technology, Kindai University.
Abstract:
Viability of chlorine-injured E. coli O157:H7 inoculated onto shredded cabbage was evaluated during storage in air or high CO2 controlled atmospheres (CA) of 5%, 10%, and 15% at 10℃ and in a modified atmosphere packaging (MAP) at 5℃ and 10℃. When shredded cabbage was inoculated with chlorine-injured E. coli O157:H7 (% injury = 65%) and then stored in air or CA at 10℃, counts of E. coli O157:H7 increased during storage and injured E. coli O157:H7 (% injury = 34-66%) were detected on samples throughout the storage regardless of the CO2 atmosphere. When shredded cabbage inoculated with chlorine injured E. coli O157:H7 (% injury = 45-59%) were stored in a MAP using either a high or low oxygen transmission permeability (OTR) package film, the counts of E. coli O157:H7 increased during storage at 10℃ and they remained constant during storage at 5℃. Injured E. coli O157:H7 were detected on shredded cabbage at a 54-56% level in a low OTR film at 10℃ and a 73-74% level in a high OTR film at 5℃. These results indicated that chlorine-injured E. coli O157:H7 inoculated on fresh-cut cabbage exhibited different degrees of injury during storage in a high CO2 CA and MAP at 5℃ or 10℃.
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