Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Frequency-dependent Selection01:21

Frequency-dependent Selection

24.0K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
24.0K
Contact-dependent Signaling01:19

Contact-dependent Signaling

47.0K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
47.0K
Drug Dependence01:17

Drug Dependence

1.7K
Medications are typically administered to achieve therapeutic effects. Some drugs can modify an individual's mood and perception, frequently resulting in various enjoyable experiences. However, this can result in drug dependency, a condition marked by continuous drug use despite potential negative consequences. Drug dependency primarily falls into two categories: psychological and physical dependence. Psychological dependence occurs when the pleasurable feelings induced by the drug...
1.7K
Target Cell Response to Hormones01:22

Target Cell Response to Hormones

5.7K
Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
5.7K
Temperature Dependence on Reaction Rate02:55

Temperature Dependence on Reaction Rate

89.0K
The Collision Theory
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
89.0K
Independent and Dependent Sources01:18

Independent and Dependent Sources

2.6K
In electrical circuits, sources play a crucial role in providing power for the operation of the circuit. These sources can be broadly categorized into two types: independent and dependent.
Independent voltage or current sources supply a fixed amount of voltage or current, respectively, which is unaffected by other elements within the circuit. These are represented using specific symbols. Independent voltage sources are symbolized with polarities (+ and -), indicating the direction of the...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Localization of four class I glutaredoxins in the cytosol and the secretory pathway and characterization of their biochemical diversification.

The Plant journal : for cell and molecular biology·2024
Same author

Breakdown of Arabidopsis thaliana thioredoxins and glutaredoxins based on electrostatic similarity-Leads to common and unique interaction partners and functions.

PloS one·2023
Same author

Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation.

Antioxidants (Basel, Switzerland)·2022
Same author

Changing Perspectives from Oxidative Stress to Redox Signaling-Extracellular Redox Control in Translational Medicine.

Antioxidants (Basel, Switzerland)·2022
Same author

An Effective Mouse Model of Unilateral Renal Ischemia-Reperfusion Injury.

Journal of visualized experiments : JoVE·2021
Same author

Molecular Basis for the Interactions of Human Thioredoxins with Their Respective Reductases.

Oxidative medicine and cellular longevity·2021

Related Experiment Video

Updated: Jan 31, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

551

Nucleoredoxin-Dependent Targets and Processes in Neuronal Cells.

Claudia Urbainsky1, Rolf Nölker2, Marcel Imber3

  • 1The Institute for Medical Biochemistry and Molecular Biology, University Medicine, University of Greifswald, Germany.

Oxidative Medicine and Cellular Longevity
|December 26, 2018
PubMed
Summary

Nucleoredoxin (Nrx), a thioredoxin family protein, acts as a cellular oxidase, regulating protein thiols and impacting metabolic pathways, cellular morphology, and signal transduction in neuronal cells. Its functions were elucidated through proteome-wide redox state analysis and substrate trapping.

More Related Videos

Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
09:39

Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings

Published on: March 17, 2023

2.9K
Author Spotlight: Hypothalamic Neural Mechanism Insights
09:29

Author Spotlight: Hypothalamic Neural Mechanism Insights

Published on: August 4, 2023

4.8K

Related Experiment Videos

Last Updated: Jan 31, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

551
Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
09:39

Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings

Published on: March 17, 2023

2.9K
Author Spotlight: Hypothalamic Neural Mechanism Insights
09:29

Author Spotlight: Hypothalamic Neural Mechanism Insights

Published on: August 4, 2023

4.8K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Proteomics

Background:

  • Nucleoredoxin (Nrx) is an oxidoreductase within the thioredoxin protein family.
  • Nrx has been implicated in signal transduction, but a comprehensive understanding of its substrates and functions was lacking.

Purpose of the Study:

  • To comprehensively analyze the substrates and functions of Nucleoredoxin (Nrx).
  • To investigate the role of Nrx in redox regulation within neuronal cells.

Main Methods:

  • Differential thiol-labeling and quantitative mass spectrometry were used to analyze the redox state of the proteome in Nrx-depleted cells.
  • An active site mutant of Nrx (Cys208Ser) was employed for substrate trapping in mouse brain and neuronal cell culture extracts.

Main Results:

  • 171 proteins exhibited altered redox states upon Nrx depletion, with 161 showing increased reduction, indicating Nrx's role in oxidizing protein thiols.
  • Substrate trapping identified 1710 proteins in mouse brain and 609 in neuronal cell cultures interacting with Nrx.
  • Nrx was found to be involved in the redox regulation of metabolic pathways, cellular morphology, and signal transduction.

Conclusions:

  • Nucleoredoxin (Nrx) functions as a cellular oxidase, potentially forming disulfide relays with peroxiredoxins.
  • These findings provide a comprehensive view of Nrx's substrates and diverse roles in cellular redox homeostasis and signaling.