Sensing Cell Membrane Biophysical Properties for Detection of High Quality Human Oocytes

Oocyte quality plays a crucial role in the early development and implantation of the embryos, and consequently has a profound impact on the accomplishment of assisted reproductive technology (ART). A simple and efficient method for detecting high-quality human oocytes is urgently needed. However, the clinically used morphological method is time-consuming, subjective, and inaccurate. To this end, we propose a practical and effective approach for detecting high-quality oocytes via on-chip measurement of the oocyte membrane permeability. We found that oocytes can be divided into two subpopulations (high-quality versus poor-quality oocytes) according to their membrane permeability differences, and as was further confirmed by subsequent in vitro fertilization (IVF) and development experiments (the blastocyst rates of high-quality and poor-quality oocytes were 60% and 0%, respectively). This approach shows great potentials in improving the success of ART, including both the fertilization and development rates, and thus it may have wide applications in the clinic.