Related Experiment Video

Updated: Jan 31, 2026

Investigating Intestinal Inflammation in DSS-induced Model of IBD
08:43

Investigating Intestinal Inflammation in DSS-induced Model of IBD

Published on: February 1, 2012

68.9K

SRC-3 protects intestine from DSS-induced colitis by inhibiting inflammation and promoting goblet cell

Wenbo Chen1,2, Minghui Zhuo2, Xuqiang Lu2

  • 1Department of Cardiology, The First Affiliated Hospital of Xiamen University, Xiamen, China.

International Journal of Biological Sciences
|December 27, 2018
PubMed

Insights

Steroid receptor coactivator 3 (SRC-3) deficiency worsens dextran sulfate sodium (DSS)-induced colitis in mice. SRC-3 promotes goblet cell differentiation and reduces inflammation by enhancing KLF4 expression, crucial for intestinal homeostasis.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Immunology

Background:

  • Goblet cell loss and reduced mucin secretion characterize ulcerative colitis (UC).
  • Steroid receptor coactivator 3 (SRC-3) plays a role in host defense and intestinal homeostasis.
  • The specific role of SRC-3 in UC pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the biological role of SRC-3 in dextran sulfate sodium (DSS)-induced colitis, a model for UC.
  • To elucidate the molecular mechanisms by which SRC-3 influences intestinal inflammation and goblet cell function.

Main Methods:

  • Utilized SRC-3 knockout (SRC-3-/-) and wild-type mice in a DSS-induced colitis model.
  • Administered 2% DSS in drinking water to induce colitis.
  • Assessed mortality, body weight loss, clinical scores, colonic histopathology, goblet cell counts, and proinflammatory cytokine levels.
  • Analyzed the expression of transcription factor KLF4 and its regulation by SRC-3 and c-Fos.

Main Results:

  • SRC-3-/- mice exhibited increased susceptibility to DSS-induced colitis, with higher mortality, weight loss, and clinical scores.
  • These mice showed severe loss of colonic goblet cells, exacerbated histopathology, and elevated proinflammatory cytokine production.
  • SRC-3-/- mice displayed decreased colonic expression of KLF4, a key regulator of goblet cell differentiation.
  • SRC-3 was found to cooperate with c-Fos to transcriptionally enhance KLF4 expression.

Conclusions:

  • SRC-3 ameliorates DSS-induced colitis by inhibiting inflammation and promoting goblet cell differentiation and maturation.
  • This protective effect is mediated through SRC-3's enhancement of KLF4 expression, crucial for maintaining intestinal barrier function.
  • SRC-3 is a critical factor in intestinal homeostasis and warrants further investigation for UC therapeutic strategies.
Keywords:
SRC-3goblet cellulcerative colitis

More Related Videos

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model09:11

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Published on: February 14, 2021

10.6K
Murine Colitis Modeling using Dextran Sulfate Sodium DSS08:39

Murine Colitis Modeling using Dextran Sulfate Sodium DSS

Published on: January 19, 2010

51.0K

Related Concept Videos

Inflammation01:38

Inflammation

Overview
62.1K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.7K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.5K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.0K